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2 changes: 1 addition & 1 deletion 3d_monolayer/README.md
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* Fiji > Image > Show Info… (Ctrl + I)
* Search for something like “Voxel size” or record this metadata when collecting your own images
* The actual units do not matter, rather their relative proportion. The numbers are unitless and therefore the decimal place does not matter.
* For this example, the relative pixel spacing is 0.065 in x and y and 0.29 pixels in z.
* For this example, the relative pixel spacing is 0.26 in x and y and 0.29 pixels in z.
1. Create "rule criteria" to identify an image by its color/channel. For example, using the Metadata you just extracted - `Metadata -> Does ->
Have ChannelNumber matching -> 0` would match the first image.
1. Give the images "variable names" that describe the contents in the image. For example, use the name *dna* or *dapi* to describe an image stained with DAPI.
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