diff --git a/samples/bluetooth/hci_uart/README.rst b/samples/bluetooth/hci_uart/README.rst
index 85b1cfa86fe8d..335a812c56a17 100644
--- a/samples/bluetooth/hci_uart/README.rst
+++ b/samples/bluetooth/hci_uart/README.rst
@@ -143,3 +143,8 @@ You can use following targets:
 * ``nrf52833dk_nrf52833@df``
 
 Check the :ref:`bluetooth_direction_finding_connectionless_rx` and the :ref:`bluetooth_direction_finding_connectionless_tx` for more details.
+
+Using a USB CDC ACM UART
+========================
+
+The sample can be configured to use a USB UART instead. See :zephyr_file:`samples/bluetooth/hci_uart/nrf52840dongle_nrf52840.conf` and :zephyr_file:`samples/bluetooth/hci_uart/nrf52840dongle_nrf52840.overlay`.
diff --git a/samples/bluetooth/hci_uart/boards/nrf52840dongle_nrf52840.conf b/samples/bluetooth/hci_uart/boards/nrf52840dongle_nrf52840.conf
new file mode 100644
index 0000000000000..b805adbec27f6
--- /dev/null
+++ b/samples/bluetooth/hci_uart/boards/nrf52840dongle_nrf52840.conf
@@ -0,0 +1,3 @@
+CONFIG_USB_DEVICE_STACK=y
+CONFIG_USB_DEVICE_PRODUCT="Zephyr HCI UART sample"
+CONFIG_USB_CDC_ACM=y
diff --git a/samples/bluetooth/hci_uart/boards/nrf52840dongle_nrf52840.overlay b/samples/bluetooth/hci_uart/boards/nrf52840dongle_nrf52840.overlay
new file mode 100644
index 0000000000000..fc41e617a1ea5
--- /dev/null
+++ b/samples/bluetooth/hci_uart/boards/nrf52840dongle_nrf52840.overlay
@@ -0,0 +1,14 @@
+/* SPDX-License-Identifier: Apache-2.0 */
+
+&zephyr_udc0 {
+    cdc_acm_uart0: cdc_acm_uart0 {
+        compatible = "zephyr,cdc-acm-uart";
+        label = "CDC_ACM_0";
+    };
+};
+
+/ {
+    chosen {
+        zephyr,bt-c2h-uart = &cdc_acm_uart0;
+    };
+};
diff --git a/samples/bluetooth/hci_uart/src/main.c b/samples/bluetooth/hci_uart/src/main.c
index a93ab0cf52e9b..6accd9ee49768 100644
--- a/samples/bluetooth/hci_uart/src/main.c
+++ b/samples/bluetooth/hci_uart/src/main.c
@@ -20,6 +20,8 @@
 #include <zephyr/init.h>
 #include <zephyr/drivers/uart.h>
 
+#include <zephyr/usb/usb_device.h>
+
 #include <zephyr/net/buf.h>
 #include <zephyr/bluetooth/bluetooth.h>
 #include <zephyr/bluetooth/l2cap.h>
@@ -328,6 +330,13 @@ static int hci_uart_init(const struct device *unused)
 {
 	LOG_DBG("");
 
+	if (IS_ENABLED(CONFIG_USB_CDC_ACM)) {
+		if (usb_enable(NULL)) {
+			LOG_ERR("Failed to enable USB");
+			return -EINVAL;
+		}
+	}
+
 	if (!device_is_ready(hci_uart_dev)) {
 		LOG_ERR("HCI UART %s is not ready", hci_uart_dev->name);
 		return -EINVAL;