diff --git a/src/lists.rs b/src/lists.rs
index c4bd21869fb..a14d1907a8c 100644
--- a/src/lists.rs
+++ b/src/lists.rs
@@ -279,10 +279,7 @@ where
                 }
 
                 if last && formatting.ends_with_newline {
-                    match formatting.trailing_separator {
-                        SeparatorTactic::Always | SeparatorTactic::Vertical => separate = true,
-                        _ => (),
-                    }
+                    separate = formatting.trailing_separator != SeparatorTactic::Never;
                 }
 
                 if line_len > 0 {
diff --git a/tests/source/macros.rs b/tests/source/macros.rs
index 6e730fffbf6..cd45b7a5d3f 100644
--- a/tests/source/macros.rs
+++ b/tests/source/macros.rs
@@ -373,3 +373,10 @@ fn foo() {
 fn foo() {
     let _ = column!(/* here */);
 }
+
+// #2616
+// Preserve trailing comma when using mixed layout for macro call.
+fn foo() {
+    foo!(1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1);
+    foo!(1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,);
+}
diff --git a/tests/target/macros.rs b/tests/target/macros.rs
index d430f5711dd..76e538bb85d 100644
--- a/tests/target/macros.rs
+++ b/tests/target/macros.rs
@@ -948,3 +948,16 @@ fn foo() {
 fn foo() {
     let _ = column!(/* here */);
 }
+
+// #2616
+// Preserve trailing comma when using mixed layout for macro call.
+fn foo() {
+    foo!(
+        1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,
+        1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
+    );
+    foo!(
+        1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,
+        1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,
+    );
+}