diff --git a/mypy/checker.py b/mypy/checker.py
index de98fa0fa179..2f2f6036bfb5 100644
--- a/mypy/checker.py
+++ b/mypy/checker.py
@@ -2072,6 +2072,24 @@ def visit_class_def(self, defn: ClassDef) -> None:
                     self.allow_abstract_call = old_allow_abstract_call
                 # TODO: Apply the sig to the actual TypeInfo so we can handle decorators
                 # that completely swap out the type.  (e.g. Callable[[Type[A]], Type[B]])
+        if typ.defn.type_vars:
+            for base_inst in typ.bases:
+                for base_tvar, base_decl_tvar in zip(
+                    base_inst.args, base_inst.type.defn.type_vars
+                ):
+                    if (
+                        isinstance(base_tvar, TypeVarType)
+                        and base_tvar.variance != INVARIANT
+                        and isinstance(base_decl_tvar, TypeVarType)
+                        and base_decl_tvar.variance != base_tvar.variance
+                    ):
+                        self.fail(
+                            f'Variance of TypeVar "{base_tvar.name}" incompatible '
+                            "with variance in parent type",
+                            context=defn,
+                            code=codes.TYPE_VAR,
+                        )
+
         if typ.is_protocol and typ.defn.type_vars:
             self.check_protocol_variance(defn)
         if not defn.has_incompatible_baseclass and defn.info.is_enum:
diff --git a/mypy/typeshed/stdlib/_typeshed/__init__.pyi b/mypy/typeshed/stdlib/_typeshed/__init__.pyi
index 89ca9d81619a..740727c69e9d 100644
--- a/mypy/typeshed/stdlib/_typeshed/__init__.pyi
+++ b/mypy/typeshed/stdlib/_typeshed/__init__.pyi
@@ -118,7 +118,7 @@ class SupportsKeysAndGetItem(Protocol[_KT, _VT_co]):
     def __getitem__(self, __k: _KT) -> _VT_co: ...
 
 # stable
-class SupportsGetItem(Container[_KT_contra], Protocol[_KT_contra, _VT_co]):
+class SupportsGetItem(Container[_KT_contra], Protocol[_KT_contra, _VT_co]):  # type: ignore
     def __getitem__(self, __k: _KT_contra) -> _VT_co: ...
 
 # stable
diff --git a/test-data/unit/check-generic-subtyping.test b/test-data/unit/check-generic-subtyping.test
index bd1f487bc895..1f06bc7c540a 100644
--- a/test-data/unit/check-generic-subtyping.test
+++ b/test-data/unit/check-generic-subtyping.test
@@ -1033,3 +1033,21 @@ x2: X2[str, int]
 reveal_type(iter(x2))  # N: Revealed type is "typing.Iterator[builtins.int]"
 reveal_type([*x2])  # N: Revealed type is "builtins.list[builtins.int]"
 [builtins fixtures/dict.pyi]
+
+[case testIncompatibleVariance]
+from typing import TypeVar, Generic
+T = TypeVar('T')
+T_co = TypeVar('T_co', covariant=True)
+T_contra = TypeVar('T_contra', contravariant=True)
+
+class A(Generic[T_co]): ...
+class B(A[T_contra], Generic[T_contra]): ...  # E: Variance of TypeVar "T_contra" incompatible with variance in parent type
+
+class C(Generic[T_contra]): ...
+class D(C[T_co], Generic[T_co]): ...  # E: Variance of TypeVar "T_co" incompatible with variance in parent type
+
+class E(Generic[T]): ...
+class F(E[T_co], Generic[T_co]): ...  # E: Variance of TypeVar "T_co" incompatible with variance in parent type
+
+class G(Generic[T]): ...
+class H(G[T_contra], Generic[T_contra]): ...  # E: Variance of TypeVar "T_contra" incompatible with variance in parent type
diff --git a/test-data/unit/fixtures/typing-full.pyi b/test-data/unit/fixtures/typing-full.pyi
index dad30dd7bcee..c406da986818 100644
--- a/test-data/unit/fixtures/typing-full.pyi
+++ b/test-data/unit/fixtures/typing-full.pyi
@@ -160,8 +160,8 @@ class SupportsAbs(Protocol[T_co]):
 def runtime_checkable(cls: T) -> T:
     return cls
 
-class ContextManager(Generic[T]):
-    def __enter__(self) -> T: pass
+class ContextManager(Generic[T_co]):
+    def __enter__(self) -> T_co: pass
     # Use Any because not all the precise types are in the fixtures.
     def __exit__(self, exc_type: Any, exc_value: Any, traceback: Any) -> Any: pass