diff --git a/Lib/test/test_venv.py b/Lib/test/test_venv.py
index eb9227a3b70379..a60f4cd3111ea8 100644
--- a/Lib/test/test_venv.py
+++ b/Lib/test/test_venv.py
@@ -495,7 +495,7 @@ def test_multiprocessing_recursion(self):
         envpy = os.path.join(os.path.realpath(self.env_dir),
                              self.bindir, self.exe)
         script = os.path.join(TEST_HOME_DIR, '_test_venv_multiprocessing.py')
-        subprocess.check_call([envpy, script])
+        subprocess.check_call([envpy, "-I", script])
 
     @unittest.skipIf(os.name == 'nt', 'not relevant on Windows')
     def test_deactivate_with_strict_bash_opts(self):