diff --git a/news/2 Fixes/17546.md b/news/2 Fixes/17546.md
new file mode 100644
index 000000000000..877e68aed6de
--- /dev/null
+++ b/news/2 Fixes/17546.md	
@@ -0,0 +1,2 @@
+Fix for `pytest` run all tests when using `pytest.ini` and `cwd`.
+(thanks [Brian Rutledge](https://github.com/bhrutledge))
diff --git a/src/client/testing/testController/pytest/runner.ts b/src/client/testing/testController/pytest/runner.ts
index c74ec5745970..96f51cfabaf6 100644
--- a/src/client/testing/testController/pytest/runner.ts
+++ b/src/client/testing/testController/pytest/runner.ts
@@ -97,7 +97,7 @@ export class PytestRunner implements ITestsRunner {
             if (!rawData) {
                 throw new Error(`Trying to run unknown node: ${testNode.id}`);
             }
-            if (testNode.id !== options.workspaceFolder.fsPath) {
+            if (testNode.id !== options.cwd) {
                 testArgs.push(rawData.rawId);
             }