diff --git a/eng/targets/Helix.Common.props b/eng/targets/Helix.Common.props
index 921b38f23564..d2acb1595a0d 100644
--- a/eng/targets/Helix.Common.props
+++ b/eng/targets/Helix.Common.props
@@ -1,7 +1,7 @@
 <Project>
   <!-- This file is shared between Helix.proj and .csproj files. -->
   <PropertyGroup>
-    <HelixQueueAlpine314>(Alpine.314.Amd64.Open)Ubuntu.2004.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:alpine-3.14-helix-amd64</HelixQueueAlpine314>
+    <HelixQueueAlpine316>(Alpine.316.Amd64.Open)Ubuntu.2004.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:alpine-3.16-helix-amd64</HelixQueueAlpine316>
     <HelixQueueDebian11>(Debian.11.Amd64.Open)Ubuntu.2004.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:debian-11-helix-amd64</HelixQueueDebian11>
     <HelixQueueFedora34>(Fedora.34.Amd64.Open)Ubuntu.2004.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:fedora-34-helix</HelixQueueFedora34>
     <HelixQueueMariner>(Mariner)Ubuntu.2004.Amd64.Open@mcr.microsoft.com/dotnet-buildtools/prereqs:cbl-mariner-1.0-helix</HelixQueueMariner>
@@ -40,7 +40,7 @@
         <HelixAvailableTargetQueue Include="Redhat.7.Amd64.Open" Platform="Linux" />
 
         <!-- Containers -->
-        <HelixAvailableTargetQueue Include="$(HelixQueueAlpine314)" Platform="Linux" />
+        <HelixAvailableTargetQueue Include="$(HelixQueueAlpine316)" Platform="Linux" />
         <HelixAvailableTargetQueue Include="$(HelixQueueDebian11)" Platform="Linux" />
         <HelixAvailableTargetQueue Include="$(HelixQueueFedora34)" Platform="Linux" />
         <HelixAvailableTargetQueue Include="$(HelixQueueMariner)" Platform="Linux" />
diff --git a/eng/targets/Helix.targets b/eng/targets/Helix.targets
index 84eaa3bba42c..adbb1c2841fe 100644
--- a/eng/targets/Helix.targets
+++ b/eng/targets/Helix.targets
@@ -16,7 +16,7 @@
 
   <PropertyGroup Condition="'$(TestDependsOnPlaywright)' == 'true'">
     <SkipHelixQueues>
-      $(HelixQueueAlpine314);
+      $(HelixQueueAlpine316);
       $(HelixQueueDebian11);
       $(HelixQueueFedora34);
       $(HelixQueueMariner);