diff --git a/.pipelines/cni/cilium/cilium-overlay-load-test-template.yaml b/.pipelines/cni/cilium/cilium-overlay-load-test-template.yaml
index 98a2305c46..a58bd03dbf 100644
--- a/.pipelines/cni/cilium/cilium-overlay-load-test-template.yaml
+++ b/.pipelines/cni/cilium/cilium-overlay-load-test-template.yaml
@@ -266,6 +266,7 @@ stages:
               fi
             name: "testAsyncDelete"
             displayName: "Verify Async Delete when CNS is down"
+          - template: ../../templates/cilium-mtu-check.yaml
       - template: ../k8s-e2e/k8s-e2e-job-template.yaml
         parameters:
           sub: $(BUILD_VALIDATIONS_SERVICE_CONNECTION)
diff --git a/.pipelines/singletenancy/cilium-dualstack-overlay/cilium-dualstackoverlay-e2e-step-template.yaml b/.pipelines/singletenancy/cilium-dualstack-overlay/cilium-dualstackoverlay-e2e-step-template.yaml
index 9337ef48d5..7c1ec21f33 100644
--- a/.pipelines/singletenancy/cilium-dualstack-overlay/cilium-dualstackoverlay-e2e-step-template.yaml
+++ b/.pipelines/singletenancy/cilium-dualstack-overlay/cilium-dualstackoverlay-e2e-step-template.yaml
@@ -161,3 +161,5 @@ steps:
       fi
     name: "testAsyncDelete"
     displayName: "Verify Async Delete when CNS is down"
+  
+  - template: ../../templates/cilium-mtu-check.yaml
diff --git a/.pipelines/singletenancy/cilium-dualstack-overlay/cilium-dualstackoverlay-e2e.steps.yaml b/.pipelines/singletenancy/cilium-dualstack-overlay/cilium-dualstackoverlay-e2e.steps.yaml
index dc49686b13..7f00f3e124 100644
--- a/.pipelines/singletenancy/cilium-dualstack-overlay/cilium-dualstackoverlay-e2e.steps.yaml
+++ b/.pipelines/singletenancy/cilium-dualstack-overlay/cilium-dualstackoverlay-e2e.steps.yaml
@@ -170,3 +170,5 @@ steps:
       fi
     name: "testAsyncDelete"
     displayName: "Verify Async Delete when CNS is down"
+  
+  - template: ../../templates/cilium-mtu-check.yaml
diff --git a/.pipelines/singletenancy/cilium-overlay-withhubble/cilium-overlay-e2e-step-template.yaml b/.pipelines/singletenancy/cilium-overlay-withhubble/cilium-overlay-e2e-step-template.yaml
index 7a81685b85..a9f024ae15 100644
--- a/.pipelines/singletenancy/cilium-overlay-withhubble/cilium-overlay-e2e-step-template.yaml
+++ b/.pipelines/singletenancy/cilium-overlay-withhubble/cilium-overlay-e2e-step-template.yaml
@@ -199,6 +199,8 @@ steps:
     name: "testAsyncDelete"
     displayName: "Verify Async Delete when CNS is down"
 
+  - template: ../../templates/cilium-mtu-check.yaml
+
   - script: |
       ARTIFACT_DIR=$(Build.ArtifactStagingDirectory)/test-output/
       echo $ARTIFACT_DIR
diff --git a/.pipelines/singletenancy/cilium-overlay-withhubble/cilium-overlay-e2e.steps.yaml b/.pipelines/singletenancy/cilium-overlay-withhubble/cilium-overlay-e2e.steps.yaml
index 6856847c1e..bdedaa7901 100644
--- a/.pipelines/singletenancy/cilium-overlay-withhubble/cilium-overlay-e2e.steps.yaml
+++ b/.pipelines/singletenancy/cilium-overlay-withhubble/cilium-overlay-e2e.steps.yaml
@@ -196,6 +196,8 @@ steps:
       fi
     name: "testAsyncDelete"
     displayName: "Verify Async Delete when CNS is down"
+  
+  - template: ../../templates/cilium-mtu-check.yaml
 
   - script: |
       ARTIFACT_DIR=$(Build.ArtifactStagingDirectory)/test-output/
diff --git a/.pipelines/singletenancy/cilium-overlay/cilium-overlay-e2e-step-template.yaml b/.pipelines/singletenancy/cilium-overlay/cilium-overlay-e2e-step-template.yaml
index 7444ef1d98..04730a16f0 100644
--- a/.pipelines/singletenancy/cilium-overlay/cilium-overlay-e2e-step-template.yaml
+++ b/.pipelines/singletenancy/cilium-overlay/cilium-overlay-e2e-step-template.yaml
@@ -248,3 +248,5 @@ steps:
       fi
     name: "testAsyncDelete"
     displayName: "Verify Async Delete when CNS is down"
+
+  - template: ../../templates/cilium-mtu-check.yaml
\ No newline at end of file
diff --git a/.pipelines/singletenancy/cilium-overlay/cilium-overlay-e2e.steps.yaml b/.pipelines/singletenancy/cilium-overlay/cilium-overlay-e2e.steps.yaml
index c82bafb9a8..39c2cae2a3 100644
--- a/.pipelines/singletenancy/cilium-overlay/cilium-overlay-e2e.steps.yaml
+++ b/.pipelines/singletenancy/cilium-overlay/cilium-overlay-e2e.steps.yaml
@@ -246,3 +246,7 @@ steps:
       fi
     name: "testAsyncDelete"
     displayName: "Verify Async Delete when CNS is down"
+  
+  - template: ../../templates/cilium-mtu-check.yaml
+  
+
diff --git a/.pipelines/templates/cilium-mtu-check.yaml b/.pipelines/templates/cilium-mtu-check.yaml
new file mode 100644
index 0000000000..6cf1967493
--- /dev/null
+++ b/.pipelines/templates/cilium-mtu-check.yaml
@@ -0,0 +1,7 @@
+steps:
+  - script: |
+      cd hack/scripts
+      chmod +x cilium-mtu-validation.sh
+      ./cilium-mtu-validation.sh
+    name: "CiliumMTUValidation"
+    displayName: "Run Cilium MTU Validation"
\ No newline at end of file
diff --git a/.pipelines/templates/cilium-tests.yaml b/.pipelines/templates/cilium-tests.yaml
index 6821e4ec56..e3d091bd3f 100644
--- a/.pipelines/templates/cilium-tests.yaml
+++ b/.pipelines/templates/cilium-tests.yaml
@@ -85,3 +85,5 @@ steps:
       fi
     name: "testAsyncDelete"
     displayName: "Verify Async Delete when CNS is down"
+  
+  - template: ./cilium-mtu-check.yaml
diff --git a/hack/manifests/nginx.yaml b/hack/manifests/nginx.yaml
new file mode 100644
index 0000000000..2ecfc95a72
--- /dev/null
+++ b/hack/manifests/nginx.yaml
@@ -0,0 +1,29 @@
+apiVersion: apps/v1
+kind: Deployment
+metadata:
+  name: nginx
+  labels:
+    app: nginx
+  namespace: kube-system
+spec:
+  replicas: 4
+  selector:
+    matchLabels:
+      app: nginx
+  template:
+    metadata:
+      labels:
+        app: nginx
+    spec:
+      containers:
+      - name: nginx
+        image: mcr.microsoft.com/azurelinux/base/nginx:1
+        ports:
+          - containerPort: 80
+      topologySpreadConstraints:
+      - maxSkew: 1
+        topologyKey: kubernetes.io/hostname # KV: Key is hostname, value is each unique nodename
+        whenUnsatisfiable: ScheduleAnyway
+        labelSelector:
+          matchLabels:
+            app: nginx
\ No newline at end of file
diff --git a/hack/scripts/cilium-mtu-validation.sh b/hack/scripts/cilium-mtu-validation.sh
new file mode 100755
index 0000000000..9e8386b849
--- /dev/null
+++ b/hack/scripts/cilium-mtu-validation.sh
@@ -0,0 +1,103 @@
+#!/bin/bash
+NAMESPACE="kube-system"
+
+echo "Deploy nginx pods for MTU testing"
+kubectl apply -f ../manifests/nginx.yaml
+kubectl wait --for=condition=available --timeout=60s -n $NAMESPACE deployment/nginx
+
+# Check node count
+node_count=$(kubectl get nodes --no-headers | wc -l)
+
+# in CNI release test scenario scale deployments to 3 * node count to get replicas on each node
+if [ "$node_count" -gt 1 ]; then
+    echo "Scaling nginx deployment to $((3 * node_count)) replicas"
+    kubectl scale deployment nginx --replicas=$((3 * node_count)) -n $NAMESPACE
+fi
+# Wait for nginx pods to be ready
+kubectl wait --for=condition=available --timeout=60s -n $NAMESPACE deployment/nginx
+
+
+
+echo "Checking MTU for pods in namespace: $NAMESPACE using Cilium agent and nginx MTU"
+
+# Get all nodes
+nodes=$(kubectl get nodes -o jsonpath='{.items[*].metadata.name}')
+
+for node in $nodes; do
+    echo "Checking node: $node"
+
+    # Get the Cilium agent pod running on this node
+    cilium_pod=$(kubectl get pods -n $NAMESPACE -o wide --field-selector spec.nodeName=$node -l k8s-app=cilium -o jsonpath='{.items[0].metadata.name}')
+
+    if [ -z "$cilium_pod" ]; then
+        echo "Failed to find Cilium agent pod on node $node"
+        echo "##[error]Failed to find Cilium agent pod on node $node"
+        exit 1
+    fi
+
+    # Get the MTU of eth0 in the Cilium agent pod
+    cilium_mtu=$(kubectl exec -n $NAMESPACE $cilium_pod -- cat /sys/class/net/eth0/mtu 2>/dev/null)
+
+    if [ -z "$cilium_mtu" ]; then
+        echo "Failed to get MTU from Cilium agent pod on node $node"
+        echo "##[error]Failed to get MTU from Cilium agent pod on node $node"
+        exit 1
+    fi
+
+    echo "Cilium agent eth0 MTU: $cilium_mtu"
+
+    # Get an nginx pod running on this node
+    nginx_pod=$(kubectl get pods -n $NAMESPACE -o wide --field-selector spec.nodeName=$node -l app=nginx -o jsonpath='{.items[0].metadata.name}')
+    if [ -z "$nginx_pod" ]; then
+        echo "Failed to find nginx pod on node $node"
+        echo "##[error]Failed to find nginx pod on node $node"
+        exit 1
+    fi
+    # Get the MTU of eth0 in the nginx pod
+    nginx_mtu=$(kubectl exec -n $NAMESPACE $nginx_pod -- cat /sys/class/net/eth0/mtu 2>/dev/null)
+    if [ -z "$nginx_mtu" ]; then
+        echo "Failed to get MTU from nginx pod on node $node"
+        echo "##[error]Failed to get MTU from nginx pod on node $node"
+        exit 1
+    fi
+    echo "Nginx pod eth0 MTU: $nginx_mtu"
+
+    # Get the node's eth0 MTU
+    node_mtu=$(kubectl debug node/$node -it --image=busybox -- sh -c "cat /sys/class/net/eth0/mtu" 2>/dev/null | tail -n 1)
+
+    if [ -z "$node_mtu" ]; then
+        echo "Failed to get MTU from node $node"
+        echo "##[error]Failed to get MTU from node $node"
+        exit 1
+    fi
+    echo "Node eth0 MTU: $node_mtu"
+
+    # Check if the MTUs match
+    if [ "$cilium_mtu" -eq "$nginx_mtu" ] && [ "$nginx_mtu" -eq "$node_mtu" ]; then
+        echo "MTU validation passed for node $node"
+    else
+        echo "MTU validation failed for node $node"
+        echo "Cilium agent MTU: $cilium_mtu, Nginx pod MTU: $nginx_mtu, Node MTU: $node_mtu"
+        echo "##[error]MTU validation failed. MTUs do not match."
+        exit 1
+    fi
+
+    echo "----------------------------------------"
+
+done
+
+# Clean up
+kubectl delete deployment nginx -n $NAMESPACE
+echo "Cleaned up nginx deployment"
+
+# Clean up the debug pod
+debug_pod=$(kubectl get pods -o name | grep "node-debugger")
+if [ -n "$debug_pod" ]; then
+    kubectl delete $debug_pod
+    kubectl wait --for=delete $debug_pod --timeout=60s
+    if [ $? -ne 0 ]; then
+        echo "Failed to clean up debug pod $debug_pod"
+    fi
+else
+    echo "No debug pod found"
+fi
\ No newline at end of file